大麦与谷类科学

为鉴定和筛选云南省抗穗发芽青稞种质资源，对36份青稞资源进行整穗发芽试验、籽粒发芽试验和休眠期鉴定等相关研究，采用穗发芽抗性分级、平均隶属函数和聚类分析等数据分析方法进行穗发芽抗性评价。在整穗发芽试验中，36份青稞材料在穗发芽率和穗发芽度上均表现出差异，穗发芽率变化范围在4.17%～100.00%，穗发芽度变化范围在2.50%～88.94%；籽粒发芽率变异幅度在4.00%～100.00%，隶属函数平均值在0.087 6～0.800 0；综合评价结果表明，参试的36份青稞材料中共筛选出13份抗性较好的材料，包括高抗1份（云青602）、抗3份（云稞4号、川12259、20QK-97）、中抗9份（云大麦12号、滇青1号、滇青2号、滇青3号、迪青6号、中甸短白青稞、15YD-3、20QK-5、20QK-3），为云南青稞抗穗发芽育种提供了宝贵的材料，也为后续的青稞抗性资源筛选提供了参考方法。

To identify and screen the highland barley germplasm resources with resistance to pre-harvest sprouting in Yunnan Province,this study conducted whole-spike germination tests, seed germination tests and dormancy period identification on 36 highland barley resources. Data analysis methods such as pre-harvest sprouting resistance grading, average membership function and cluster analysis were used to evaluate the pre-harvest sprouting resistance. In the spike germination resistance classification experiment and whole-spike germination test, the 36 hulless barley(Hordeum vulgare L. var. nudum) materials exhibited significant variations in both spike germination rate(ranging from 4.17% to 100.00%) and spike germination degree(2.50% to 88.94%). The grain germination rate displayed a similar broad variability spectrum(4.00% to 100.00%). The membership function average values for comprehensive evaluation of sprouting resistance ranged from 0.087 6 to 0.800 0. The comprehensive evaluation results showed that among the 36highland barley materials tested, 13 materials with better resistance were screened out, including 1 highly resistant material(Yunqing602), 3 resistant materials(Yunke No.4, Chuan 12259, 20QK-97), and 9 moderately resistant materials(Yundamai No.12, Dianqing No.1, Dianqing No.2, Dianqing No.3, Diqing No.6, Zhongdian short white highland barley, 15YD-3, 20QK-5, 20QK-3), which can provide valuable materials for future highland barley pre-harvest sprouting resistance breeding in Yunnan Province and a reference method for subsequent screening of resistant resources.